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In order to understand molecular phylogenetics of silkworm (Bambyx mori), we analyzed the sequences of BmoMAR isolated from Bmobyx mori that is partial coding gene of transpoasae of mariner-like element(MLE). By pairwise comparing nucleotide sequences of BmoMAR with ten prewiously reported insect MLEs accessed in GeneBank, the average genetic distance was estimated to be 0.4840. The phylogenetics tree constructed from nine insect species except for human MLE(Hamar1) by UPGMA method indicated that MLEs are divided into three clusters, and Drosophilia mariutiana was independently subgrouped. Bombyx mori(BmoMAR) was subgrouped with microcaddishfly (Orthotrichia cristata), webworm(Atteva punctella), almond moth(Ephestia cautella), Hyalopora cecropia which are lepidoptera. Phylogenetics tree according to UPGMA principle, on the basis of informative nucleotide sequences of nine insect MLEs, idicated that Bombyx mori was more closely related to microcaddishfly(Orthotrichia cristata) and wdbworm (Atteva punctella)of lepidoptera. We suggest that insect MLEs are a useful ket for studying molecular phylogenetics among intra species of insects.
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